TABLE 1.

Comparison of experimental peptide masses obtained from the spectra shown in Fig. 3 with theoretical peptide masses obtained from virtual tryptic digestion of the ShmR protein from S. meliloti 1021

Peptide sequenceTheoretical mass (Da)Exptl mass (Da)Difference (Da)
G109-R114586.33586.330.00
L55-R60615.35615.360.01
T632-K637662.38662.380.00
V737-F743a850.47850.470.00
A101-R108a905.48905.480.00
I706-R713a939.50939.520.02
D726-R733970.43970.42−0.01
T351-R359991.52991.51−0.01
I275-R283993.54993.52−0.02
K705-R7131,067.601,067.59−0.01
T734-R7421,079.621,079.690.07
Q81-R901,131.561,131.560.00
I275-R2841,149.631,149.62−0.01
I300-R3091,172.521,172.51−0.01
G714-R7251,187.601,187.59−0.01
S426-R4361,191.601,191.600.00
T734-F7431,226.681,226.66−0.02
F265-R2741,237.611,237.60−0.01
A334-R3431,280.621,280.61−0.01
T180-K1911,302.641,302.57−0.07
D726-K7361,346.631,346.62−0.01
V694-K7051,382.731,382.69−0.04
Y298-R3091,463.681,463.67−0.01
A101-R1141,472.801,472.74−0.06
F218-R2301,489.741,489.740.00
D726-K7361,495.671,495.66−0.01
I437-R4511,503.811,503.79−0.02
D285-R2971,515.731,515.70−0.03
F218-K2311,617.841,617.81−0.03
G543-R5581,669.781,669.76−0.02
I298-R3111,734.801,734.78−0.02
S91-R1081,875.961,875.89−0.07
T249-K2641,892.901,892.87−0.03
I115-R1321,916.041,916.02−0.02
S199-R2171,949.881,949.86−0.02
T605-K6222,004.952,004.92−0.03
D377-R3952,083.972,083.94−0.03
T180-R1982,148.062,148.03−0.03
D726-F7432,178.092,178.07−0.02
F265-R2832,212.132,212.09−0.04
V228-R3332,288.992,288.96−0.03
R694-R7132,303.212,303.19−0.02
A374-R3952,340.122,340.10−0.02
T351-R3732,468.182,468.15−0.03
V559-R5832,769.312,769.28−0.03
  • a Peptide sequenced.