TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaSourceb
E. coli DH5αF endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 ΔU169 deoRLaboratory stock
E. coli BL21B F ompT hsdS (rB mB) gal dcmAmersham Pharmacia
E. coli JM101supE thi Δ(lac-proAB) F′[tra D36 proAB+ lacIq lacZ ΔM15]Laboratory stock
S. paucimobilis B90Aerobic, motile, rod shaped; degrades all four isomers of HCH (α, β, γ, and δ)CRRI, Cuttack, India
S. paucimobilis UT26Aerobic, motile, rod shaped; degrades α, γ, and δ-HCH but not β-HCHY. Nagata
pUC 13/192.7-kb; Ampr; MCS internal to lacZ geneLaboratory stock
pGEX-5X-3GST fusion vector with tac promoter, lacIq, factor Xa protease recognition siteAmersham Pharmacia
pIMA2Recombinant construct carrying truncated linA geneY. Nagata
pYNA4RRecombinant construct carrying truncated linB geneY. Nagata
pBFRU2Recombinant construct carrying truncated linC geneY. Nagata
pKM2Recombinant construct carrying truncated linD geneY. Nagata
pLIN14pUC13 carrying 1.4-kb BclI fragment from genomic DNA of S. paucimobilis B90This study
pLIN25pUC13 carrying 2.5-kb BclI fragment from genomic DNA of S. paucimobilis B90This study
pLINA1pUC19 containing linA1 geneThis study
pLINA2pUC19 containing linA2 geneThis study
pLINBpUC19 containing linB geneThis study
pLINCpUC19 containing linC geneThis study
pLINDpUC19 containing linD geneThis study
pLINXpUC19 containing linX geneThis study
pLINEA1pGEX-5X-3 containing linA1 geneThis study
pLINEA2pGEX-5X-3 containing linA2 geneThis study
  • a MCS, multiple cloning site; GST, glutathione S-transferase.

  • b CRRI, Central Rice Research Institute.