TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionaSource or reference
E. coli
    DH5αF, φ80 lacZΔM15 endA1 recA1 hsdR17(rK mK+) supE44 thi-1 ΔgyrA96 (ΔlacZYA-argF)U169 relA1Lab stocks
Burkholderia sp.
    ATCC 25416 B. cepacia, genomovar I, environmental isolateD. P. Speert
    CEP509 B. cepacia, genomovar I, clinical isolateD. P. Speert
    Cep484 B. multivorans, clinical isolateD. P. Speert
    C5424 B. cepacia, genomovar III, clinical isolateD. P. Speert
    C4455 B. cepacia, genomovar III, clinical isolateD. P. Speert
    K56-2 B. cepacia, genomovar III, clinical isolateD. P. Speert
    CEP559 B. stabilis, clinical isolateD. P. Speert
    CEP040 B. vietnamiensis, clinical isolateD. P. Speert
    CEP021 B. cepacia, genomovar VI, clinical isolateD. P. Speert
    LB400 Burkholderia sp., environmental isolateS. Sehay
Plasmids
    pME6000pBBR1 ori, TetrlacZ mob+S. Heeb
    pML50pME6000, PS7-dhfrThis study
    pML51pME6000, promoterless dhfrThis study
    pML52pME6000, araC-PBAD-dhfrThis study
    pBAD24AraC AmprcolE1D.E. Heinrichs
    pRK2013RK2 derivative, Kmrmob+tra+ ColE1 15
    pTnMod-OTp′Plasposon, Tpr 13
    pMLS7pBBR1 ori, PS7 promoter, Tprmob+This study
    pMLBADpBBR1 ori, araC-PBAD Tprmob+This study
    pMLS7-eGFPpMLS7, e-gfpThis study
    pMLBAD-eGFPpMLBAD, e-gfpThis study
    pML53pMLBAD-eGFP, PDHFR-araEThis study
    pIJ8668e-gfp Aprr 40
  • a Ampr, ampicillin resistance; Aprr, apramycin resistance; Kmr, kanamycin resistance; Tetr, tetracycline resistance; Tpr, trimethoprim resistance.