Pressure level (MPa) | Log survivors (CFU/g) (mean ± SD) after treatment at the following temp and pressure treatment time^{b}: | |||||
---|---|---|---|---|---|---|

40°C | 45°C | 50°C | ||||

2 min | 5 min | 2 min | 5 min | 2 min | 5 min | |

250 | 3.5 ± 0.3 | ND | ND | ND | 1 ± 0.35 | ND |

300 | 2.9 ± 0.5 | ND | <0.8 (3) | <0.8 (2) | <0.8 (0) | ND |

350 | <0.8 (3) | <0.8 (2) | <0.8 (1) | <0.8 (2) | <0.8 (0) | ND |

400 | <0.8 (2) | <0.8 (1) | <0.8 (1) | <0.8 (0) | <0.8 (0) | ND |

450 | <0.8 (1) | <0.8 (1) | <0.8 (0) | <0.8 (0) | <0.8 (0) | ND |

500 | <0.8 (1) | <0.8 (1) | <0.8 (1) | <0.8 (0) | <0.8 (0) | ND |

550 | <0.8 (0) | ND | <0.8 (0) | ND | <0.8 (0) | ND |

↵

*a*The initial populations of*E. coli*O157:H7 on inoculated and dried seeds were 5.7 log CFU/g, as determined by plating appropriate dilutions on TSAYE-N.↵

*b*The number of survivors (log survivors [CFU/g]) after treatment at three temperatures for 2 or 5 min at different pressures. Data representing log survivors (CFU/g) are the means of three replicates. The numbers in parentheses are the number of samples testing positive after enrichment out of a total of three trials. ND, not done.