TABLE 2.

Probes developed and used in this study

Probe name (E. coli position)Probe sequence (5′-3′)Length (nta)Tm (°C)bOptimal formamide %Targets
APC987 (987-998)CCTAAYCATGTCCGGACC185735AMD APC group
HLB1074 (1074-1092)cAASWAGACAGATCACTCCACG215635Heterolobosea
VAH1044 (1044-1059)ACCATGCACCACCTTCTG185835Vahlkampfiidae
NAE1041 (1041-1055)TGCACTACCCACCACACA185830Naegleria spp.
DOH299 (299-317)CCTCTCCGGTATCGTACCC196010Fungus IM group II