TABLE 1.

nifH, 16S rRNA, and mcrA gene clone libraries constructed for the deep-sea methane seep sediments of the Okhotsk Sea

Sampling stationnifH16S rRNAmcrA
No. of clonesNo. of RFLP phylotypesNo. of OTUsaNo. of clonesNo. of RFLP phylotypesNo. of OTUsaNo. of clonesNo. of RFLP phylotypesNo. of OTUsa
LV39-25H794326934120931713
LV39-40H793821902214792323
  • a OTUs were calculated using DOTUR (28) based on 5% sequence distance for the NifH protein sequences, 3% sequence distance for the 16S rRNA gene sequences, and uniqueness of the McrA protein sequences.