TABLE 1.

Oligonucleotides utilized in this studya

Oligonucleotide sequenceUtility
AGAGATCACATATGTGGTGGACCGCTACCAAPCR for pFA5
CTTTTGCGCATATGCTAGGAGAACCGGCCCGGCCPCR for pFA5
GAACGCTCCGCTCCCTATCACMutagenesis of T60A
GAACGCTCCNNKCCCTATCACMutagenesis of T60X
CGGGCTACCGGCGTCGAAACCMutagenesis of A188G
CGGGCTACCNNKGTCGAAACCMutagenesis of A188X
GCGGCAGTGCTGAATACGCCGMutagenesis of M244L
GCGGCAGTGNNKAATACGCCGMutagenesis of M244X
AATCCGGGTAGCACTTTTGCCMutagenesis of N257S
AATCCGGGTNNKACTTTTGCCMutagenesis of N257X
ACTTTTGCCATGGATCACGACMutagenesis of L261M
ACTTTTGCCNNKGATCACGACMutagenesis of L261X
  • a N indicates a mixture of four nucleotides. X indicates any of 20 amino acids.