TABLE 3.

Rounds of thermostabilization of FAOXa

RoundProductivity (U/ml)% of activity at 47°C for 10 minKm value (mM)Mutation sites
Parent (pFA101, FAOX-C)0.10 ± 0.011.0 ± 0.020.74
1st (pFAH11)0.11 ± 0.0130.0 ± 0.550.57A188G M244L
2nd
    pFAH210.10 ± 0.0135.5 ± 0.590.42A188G M244L L261M
    pFAH220.11 ± 0.0170.0 ± 1.250.50T60A A188G M244L
3rd (pFAH31)0.10 ± 0.0180.0 ± 1.520.50T60A A188G M244L L261M
4th (pFAH41 FAOX-TE)0.10 ± 0.0190.0 ± 1.610.50T60A A188G M244L N257S L261M
  • a Two thermostable mutants were obtained in the second screening round. The Km value was measured for FG. The data represent the means ± standard deviations of three measurements.