TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaSource or reference
B. breve NCFB 2257NCFBb
B. lactisThis study
E. coli JM109recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 λ Δ(lac-proAB) (F′ traD36 proAB lacIqZΔM15)41
pEcoR251Apr (EcoRI)53
pMT104Apr, 0.15-kb B. fragilis fragment in pEcoR25149
pSuc1Apr, 4.7-kb Sau3A B. lactis fragment in pEcoR251This study
pSuc2Apr, 3.95-kb Sau3A B. lactis fragment in pEcoR251This study
pSuc3Apr, 5.9-kb Sau3A B. lactis fragment in pEcoR251This study
pΔST8Apr, 3.2-kb SmaI-StuI fragment from pSuc3 in pEcoR251This study
pΔReg2Apr, 3.9-kb PstI fragment from pSuc3 in pEcoR251This study
  • a Apr, resistance to ampicillin.

  • b NCFB, National Collection of Food Bacteria, Reading, United Kingdom.