TABLE 1.

Plasmids used in this study

PlasmidCharacteristicsSource or reference
pVC7Corynebacterium-E. coli shuttle vector; Cmr21
pVKTG1pVC7 carrying fused pre-transglutaminase gene containing signal peptide of CspBThis work
pVKTG2pVC7 carrying fused pre-transglutaminase gene containing N-terminal 31 amino acid residues of CspBThis work
pVKTG3pVC7 carrying fused pre-transglutaminase gene containing N-terminal 44 amino acid residues of CspBThis work
pVKPTG1pVC7 carrying fused prepro-transglutaminase gene containing signal peptide of CspBThis work
pVKPTG2pVC7 carrying fused prepro-transglutaminase gene containing N-terminal 31 amino acid residues of CspBThis work
pVKPTG3pVC7 carrying fused prepro-transglutaminase gene containing N-terminal 44 amino acid residues of CspBThis work
pVKPTG0pVC7 carrying native prepro-transglutaminase gene expressed under the control of the cspB promoterThis work
pUJP45Plasmid carrying SAM-P45 gene31
pVSS1pVC7 carrying fused prepro-SAM-P45 gene containing signal peptide of CspA of C. ammoniagenesThis work
pPK4Corynebacterium-E. coli shuttle vector; Kmr8
pPKPTG1pPK4 carrying fused prepro-transglutaminase gene containing signal peptide of CspBThis work
pPAPTG1pPK4 carrying fused prepro-transglutaminase gene containing signal peptide of CspAThis work
pPSPTG1pPK4 carrying fused prepro-transglutaminase gene containing signal peptide of CspA of C. ammoniagenesThis work
pUC19E. coli cloning vector; Apr29
pUMTG5pUC19 carrying transglutaminase gene containing 5′-flanking regionThis work
pUKPTG1pUC19 carrying fused prepro-transglutaminase gene containing signal peptide of CspBThis work
pUAPTG1pUC19 carrying fused prepro-transglutaminase gene containing signal peptide of CspAThis work
pUSPTG1pUC19 carrying fused prepro-transglutaminase gene containing signal peptide of CspA of C. ammoniagenesThis work