TABLE 4.

Thermostabilization of FAOX by site-directed mutagenesisa

Mutation site(s)% of activity at 47°C for 10 minKm (mM)
None (wild type)1.0 ± 0.020.74
T60A28.8 ± 0.560.50
A188G1.0 ± 0.020.74
M244L29.5 ± 0.600.57
N257S1.5 ± 0.040.70
L261M2.5 ± 0.050.70
T60A M244L69.0 ± 1.290.45
T60A A188G M244Lb70.0 ± 1.250.50
  • a The Km value is measured for FG. The data represent the means ± standard deviations of three measurements.

  • b Second mutant in the second screening round.