TABLE 5.

Comparison of wild-type FAOX-C and thermostable mutant FAOX-TE by using purified enzymesa

EnzymeSp act for FG (U/mg)Specificity (%)Km (mM)kcat (s−1)kcat/Km (mM−1 s−1)pI
FGFVεFf LysFGFVFGFVFGFV
FAOX-C (wild type)5.46 ± 0.11100 ± 2.1132 ± 2.500.741.618.01 ± 0.1610.60 ± 0.2110.80 ± 0.226.57 ± 0.134.6
FAOX-TE (mutant)5.32 ± 0.11100 ± 2.0124 ± 2.300.581.507.80 ± 0.169.68 ± 0.1915.60 ± 0.316.45 ± 0.134.2
  • a The data represent the means ± standard deviations of three measurements.