TABLE 1

Abundances of the top 23 families in 46 nasal specimens, analyzed by 16S rRNA-based conventional and PMA microbiome methods

Detection methodPMA pretreatmentTargetTaxon (at family level)Avg relative abundanceSDRangeNo. (%) of carrying individuals
Conventional microbiomeNoTotal DNACorynebacteriaceae0.4326360.2240330.03542–0.9403646 (100)
Staphylococcaceae0.3324920.2117700.00609–0.7023246 (100)
Peptoniphilaceae0.0783140.1280430–0.5379745 (97.8)
Carnobacteriaceae0.0573200.1423490–0.5001635 (76.1)
Neisseriaceae0.0134810.0309940–0.1703835 (76.1)
Streptococcaceae0.0064360.0156320–0.0963442 (91.3)
Prevotellaceae0.0033130.0142590–0.0957525 (54.3)
Veillonellaceae0.0028200.0055930–0.0246932 (69.6)
Moraxellaceae0.0023420.0062330–0.0313931 (67.4)
Micrococcaceae0.0020460.0041920.00009–0.0241646 (100)
Actinomycetaceae0.0018710.0053840–0.0315831 (67.4)
Rhodobacteraceae0.0018600.0091400–0.0621235 (76.1)
Bacillaceae0.0017640.0038300–0.0245744 (95.7)
Nocardioidaceae0.0016410.0104700–0.0711016 (34.8)
Comamonadaceae0.0015420.0051490–0.0266420 (43.5)
Campylobacteraceae0.0014890.0081560–0.0551712 (26.1)
Pasteurellaceae0.0014790.0060210–0.0403919 (41.3)
Nocardiaceae0.0011990.0038160–0.0255032 (69.6)
Pseudomonadaceae0.0010530.0027120–0.0153224 (52.2)
Flavobacteriaceae0.0010120.0042950–0.0283221 (45.7)
Fusobacteriaceae0.0007940.0030830–0.0153313 (28.3)
Porphyromonadaceae0.0007850.0037070–0.0247013 (28.3)
Propionibacteriaceae0.0007840.0025670–0.0175634 (73.9)
PMA microbiomeYesDNA from viable cellsStaphylococcaceae0.4352620.2670300.00353–0.8825646 (100)
Corynebacteriaceae0.3751950.2704690.02003–0.9368346 (100)
Peptoniphilaceae0.0514350.1200540–0.4842340 (87.0)
Carnobacteriaceae0.0495840.1629100–0.8160122 (47.8)
Comamonadaceae0.0047330.0119200–0.0499124 (52.2)
Pseudomonadaceae0.0040950.0126750–0.0705429 (63.0)
Methylobacteriaceae0.0038560.0106890–0.0596020 (43.5)
Rhizobiaceae0.0036890.0121180–0.0763022 (47.8)
Moraxellaceae0.0034130.0129870–0.0769517 (37.0)
Spirulinaceae0.0029520.0188910–0.128226 (13.0)
Bacillaceae0.0026440.0072640–0.0426939 (84.8)
Flavobacteriaceae0.0016850.0054800–0.0327912 (26.1)
Bradyrhizobiaceae0.0011000.0029610–0.0174819 (41.3)
Micrococcaceae0.0009590.0031400–0.0202327 (58.7)
Neisseriaceae0.0008750.0023740–0.0132615 (32.6)
Campylobacteraceae0.0008720.0059140–0.040111 (2.2)
Rhodobacteraceae0.0008390.0031420–0.0200512 (26.1)
Veillonellaceae0.0007220.0024290–0.012648 (17.4)
Caulobacteraceae0.0005730.0025450–0.016359 (19.6)
Cytophagaceae0.0005630.0037220–0.025252 (4.3)
Propionibacteriaceae0.0004710.0023880–0.0162219 (41.3)
Hymenobacteraceae0.0004630.0030330–0.020583 (6.5)
Enterobacteriaceae0.0004410.0022950–0.015046 (13.0)