TABLE 1.

Integration sites of transposons in the mutants derived from field isolate 56a

MutantIntegration siteGenomic locationDescriptionTransposon orientation
M1AAAGCTATTGTACTT-Em-Mu-TACCTAAAGATTTAT2540298-2540302Heat-inducible transcriptional repressor HrcA+
M2ACATTAAAATGTAAA-Em-Mu-GTAAAGACTGTGGAG1431407-143411Conserved hypothetical protein
M3TGTTCAGCTGACCGA-Em-Mu-ACCGATACTAATAGA77125-7712923S rRNA+
M4CTTCAGTTTAACTGA-Em-Mu-ACTGAAAGTTCTTTG3215472-3215476Intergenic
M5TTCTGCCTCTTCTGA-Em-Mu-TCTGATAGTATTAC2963631-2963635Methionine aminopeptidase, type I
M6TTACCTTTGTAGTTA-Em-Mu-AGTTACAACATCTCT332463-332467Transcriptional regulator, AbrB family
M7GTAAAGGTGTTTCAG-Em-Mu-TTCAGAAGAAAAAAT530994-530998BFD-like iron-sulfur cluster binding protein+
  • a Target site duplications are indicated by boldface T, A, C, and G. All genomic locations were determined by comparison with the complete sequence of strain ATCC 13124 (GenBank accession number NC_008261) (18). All genes described were transcribed in the sense direction. Transcription of the transposon was compared to the local transcription within the genomic location.