Primer specificity for amplification of gene fragments from L. monocytogenes compared to other species of the genus Listeria

Gene targetAnnealing temp (°C)StrainAmplicon size (bp)FluorescenceaGelb
hly58L. monocytogenes ATCC 19115273115+
L. innocua ATCC 3309128
L. ivanovii ATCC 1911927
L. grayi ATCC 2540130
L. seeligeri ATCC 3596732
L. welshimeri ATCC 3589730
Water (negative control)26
iap60L. monocytogenes ATCC 19115330189+
L. innocua ATCC 3309145
L. ivanovii ATCC 1911943
L. grayi ATCC 2540144
L. seeligeri ATCC 3596743
L. welshimeri ATCC 3589746
Water (negative control)40
  • a The values are fluorescence values (excitation wavelength, 485 nm; emission wavelength. 530 nm) obtained with a CytoFluor II plate reader.

  • b + or − indicates the presence or absence, respectively, of bands of the expected size as determined by agarose gel electrophoresis and EtBr staining.