TABLE 1.

Characteristics of Stx2-producing HUS- and bovine-associated STEC isolates

IsolateSourceStx2 type(s)SerogroupGeneaPCR product with primers forb:Total [Stx2]e (ng ml−1)
eaeehxAQ-stx2cstx2-Sd
H2AHUS patient2O157++++11,960
H2BHUS patient2, 2cO157+++26,230
H2CHUS patient2O157+++13,240
H2DHUS patient2O157+++12,840
H2EHUS patient2O157+++10,270
H2FHUS patient2O157++++11,160
H2GHUS patient2O121+++9,340
H2HHUS patient2O157+++9,650
H2IHUS patient2O157+++9,340
H2JHUS patient2O121+++9,050
H2KHUS patient2, 2cO157+++4,010
H2LHUS patient2O157+++10,870
H2MHUS patient2O157++++5,410
B2ACattle2cO6++170
B2BCattle2cO6+10
B2CCattle2O38++10
B2DCattle2cO91200
B2ECattle2, 2cO96++880
B2FCattle2O98+++10,190
B2GCattle2O113+10
B2HCattle2, 2cO113+++100
B2ICattle2, 2cO113++520
B2JCattle2cO157+3
B2KCattle2O15711,440
B2LCattle2cO157+30
B2MCattle2O163++20
B2NCattle2, 2cO165+++2,520
B2OCattle2cO171+250
B2PCattle2O172++++10,790
B2QCattle2OX3f980
B2RCattle2cOX3+130
B2SCattle2OX18++980
B2TCattle2OX25++17,110
B2UCattle2cNEGg++1,210
B2VCattle2NEG+4,990
B2WCreek water2NEG+++1,370
B2XCattle2cNEG++++1
B2YCattle2, 2cNEG+++1,300
  • a +, presence of indicated gene; −, absence of indicated gene.

  • b +, presence of indicated PCR product; −, absence of indicated PCR product.

  • c PCR product size of 900 bp between Q and stx2.

  • d PCR product size of 3,455 bp between stx2 and S.

  • e Total Stx2 concentration in mitomycin C-treated culture (see text for details).

  • f OX serogroups refer to a provisional designation for an as-yet-unclassified O group.

  • g NEG, isolates did not react with any of the 181 O antisera used in standard testing.