TABLE 1.

Toxicity of B. thuringiensis subsp. israelensis 4Q7 strains producing the B. sphaericus binary toxin, B. thuringiensis Cry11B, and/or Cyt1A against fourth-instar C. quinquefasciatus and A. aegypti

StrainToxin combinationLC50 (fiducial limits)aSlope (± SE)
C. quinquefasciatus
    4Q7/pPFT11Bs-CRPCry11B9.2 (6.8 − 12.1) a2.0 ± 0.2
    4Q7/p45S1Bin + Cyt1A3.7 (2.5 − 5.2) b1.5 ± 0.2
    4Q7/p45S1-11BCry11B + Bin + Cyt1A1.7 (1.2 − 2.5) c1.5 ± 0.2
    Bti IPS-82Cry4A + Cry4B + Cry11A + Cyt1A7.9 (6.2 − 9.7) a3.4 ± 0.6
    Bs 2362Bin12.6 (8.7 − 17.5) a1.4 ± 0.2
A. aegypti
    4Q7/pPFT11Bs-CRPCry11B28.5 (17.7 − 45.2) a2.6 ± 0.5
    4Q7/p45S1Bin + Cyt1A265.5 (214.9 − 321.6) b3.0 ± 0.4
    4Q7/p45S1-11BCry11B + Bin + Cyt1A37.2 (30.5 − 44.8) a3.0 ± 0.4
    Bti IPS-82Cry4A + Cry4B + Cry11A + Cyt1A10.2 (8.0 − 21.9) c5.6 ± 1.6
    Bs 2362BinN/Tb
  • a 24-h mortality, in nanograms/milliliter. Values followed by different letters were significantly different.

  • b N/T, not toxic at 500 ng/ml.