TABLE 1.

PCR primers used in this study

PrimerSequence (5′→3′)
gSOE-AaCGT CGA ATT CAG CCT ACG AGC
gSOE-BTCG AGC AAT TGA TCT TGC AT
gSOE-CbATG CAA GAT CAA TTG CTC GAC CTT CCG CGT ACC AAG TGT TAG
gSOE-DaTAA ATT CTA GAA GTA TTT GGA AGC
gSOE-FCTT TGG TTT ATT CCC GAA CAG AAC
gSOE-RTTT GTC ATG TAT TTT GGT ACT GC
oSOE-AaGTA ACG AAG CTT TAT AAA GGG G
oSOE-BAAT CAA GTT TTG CTC TGC
oSOE-CbGCA GAG CAA AAC TTG ATT CAA ACA ATT CCG GCA CTA GCA ATG T
oSOE-DaGCG TTG GAT CCA ACC AAG AAG T
oSOE-FTGG ACA GCA GCA ACG TAT AG
oSOE-RCAT TTA TGA TAA AAA GTT TAC TAC
bSOE-AaTTT CTA GAA AGT AAT TTT GGT TGG TAT
bSOE-BTCC CCA GTG GAA GAA TGA
bSOE-CbTCA TTC TTC CAC TGG GGA ATT TTT GTC GAA CAA CAT GGT AAT
bSOE-DaAAT CGA AGC TTT TTG AAG CGC TGT
bSOE-FAGT CCG ATT GGC TCG ATT CGA C
bSOE-RTCG CGA AAT AGT CGC GGC AAA GC
  • a Nucleotide substitutions to create restriction sites are underlined.

  • b Overhangs complementary to SOE-B primers are underlined.