TABLE 2.

Characteristics of Stx1-producing HUS- and bovine-associated STEC isolates

IsolateSourcestx1 typeSerogroupGenePCR product with primers for:Total [Stx1]e (ng ml−1) in the presence of mitomycin CTotal [Stx1]f (ng ml−1) after growth in low Fe
eaeehxAQ-stx1astx1-S
H1AHUS patientg1O26:H11++2,2301,610
H1BHUS patientg1O92:H33++++c4602,740
H1CHUS patientg1O103:H2++3705,020
H1DHUS patientg1O103:H−++6602,390
H1EHUS patientg1O118:H−+++2,4301,550
H1FHUS patientg1O145:H−++++c1,1701,320
B1AWater sample1O103+++21013,160
B1BWater sample1O103++18014,910
B1CCattle1O109++7,6901,930
B1DCattle1O109+11030
B1ECoyoteh1O111+++504,210
B1FCattle1cO121+b10100
B1GCattle1cO121210120
B1HCattle1O141++<1280
B1ICattle1cO142+b290350
B1JWater sample1cO142<110
B1KCattle1O145++9602,570
B1LCattle1O145+++d602,400
B1MWater sample1cNEGi210670
B1NCattle1cNEG+250280
B1OCattle1cNEG+b130
B1PCattle1NEG+1,3701,040
B1QWater sample1NEG++7,1501,760
B1RCattle1NEG+++22010,310
B1SWater sample1NEG+++d1203,290
B1TCattle1NEG++2906,890
  • a PCR product size of 700 bp.

  • b PCR product size of 600 bp.

  • c PCR product size of 1,000 bp.

  • d PCR product size of 3,000 bp.

  • e Total Stx1 concentration following mitomycin C treatment.

  • f Values indicate total Stx1 concentration following growth in low-iron medium.

  • g Isolates obtained from H. Schmidt (Wurzburg, Germany). Serotypes for these isolates were provided by H. Schmidt.

  • h Fecal sample from coyote found in a pasture enclosure.

  • i Isolates did not react with any of the 181 O antisera used in standard testing.