TABLE 3.

Occurrence of group II introns B.th.I.1 and B.th.I.2 in 58 B. cereus strains containing pXO2-like repA and type IV-like secretion system genes

B.th.I.2No. of strains with B.th.I.1:
PresentAbsentUnknownaTotal
Present911525
Absent115016
Unknowna012517
Total10282058
  • a Primer pairs Bth1_F1/Bth1_R2 and MD4_F1/MD4_F1R1 were designed to flank the group II introns B.th.I.1 and B.th.I.2, respectively. The presence of B.th.I.1 and B.th.I.2 was therefore determined by the size of the PCR amplicons. When a PCR product was not present, the result was inconclusive (indicated by “unknown”).