TABLE 2.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype and/or relevant characteristic(s)Source
Strains
    P. alvei CCM 2051TWild-type isolate; KmrCCM
    P. alvei CCM 2051 wsfP::Ll.LtrBP. alvei CCM 2051T carrying a targetron insertion at the wsfP locus; KmrThis study
    Escherichia coli DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK mK) phoA supE44 thi-1 gyrA96 relA1 λInvitrogen
Plasmids
    pNW33NGeobacillus-Bacillus-E. coli shuttle vector; CmrBacillus Genetic Stock Center
    pEXALVpNW33N carrying the sgsE S-layer gene promoter of G. stearothermophilus NRS 2004/3a; CmrThis study
    pNW33NΔHindIIIpNW33N without its unique HindIII restriction site; CmrThis study
    pJIR750aiClostridium perfringens-E. coli shuttle vector carrying alpha-toxin gene (plc) targetron; CmrSigma-Aldrich
    pJIR750ai_P(SgsE)pJIR750ai carrying the sgsE S-layer gene promoter of G. stearothermophilus NRS 2004/3a; CmrThis study
    pTT_plcpNW33N without HindIII carrying the sgsE S-layer gene promoter of G. stearothermophilus NRS 2004/3a in front of the plc intron cassette of pJIR750ai; CmrThis study
    pTT_wsfP555pTT_plc targeted for insertion between positions 555 and 556 from the initial ATG codon of wsfP; CmrThis study
    pTT_wsfP654pTT_plc targeted for insertion between positions 654 and 655 from the initial ATG codon of wsfP; CmrThis study
    pTT_wsfP1176pTT_plc targeted for insertion between positions 1176 and 1177 from the initial ATG codon of wsfP; CmrThis study
    pEXALV_wsfPpEXALV carrying the wsfP gene of P. alvei CCM 2051T; CmrThis study
    pEXALV_wsaPpEXALV carrying the wsaP gene of G. stearothermophilus NRS 2004/3a; CmrThis study