TABLE 2.

Efficiency of transduction and infection of S. aureus wild-type and hsdR mutant strains using phages 80α and φ75

StrainTransduction of plasmid pGC2 from strain 879R4RF (no. of transductants/106 phage particles)Efficiency of plaquinga
Phage 80αPhage φ75Phage 80α from 879R4RFPhage φ75 from 879R4RF
RN42201586201.01.0
NCTC8325-4003.1 × 10−53.7 × 10−6
NCTC8325-4ΔHsdR005.8 × 10−53.9 × 10−3
NCTC8325-4ΔC-term HsdR001.0 × 10−46.1 × 10−3
SH1000004.5 × 10−54.7 × 10−5
SH1000ΔHsdR001.6 × 10−43.6 × 10−3
  • a The efficiency of plaquing was calculated by dividing the number of phage PFU per milliliter of phage lysate in each strain tested by the corresponding number in RN4220.