TABLE 2.

Comparative gene expression profiles of NCFM and the Δupp mutant NCK1909 grown in MRS medium

Open reading frameAnnotationΔupp/NCFM expression ratioLog2 ratioP value
Gene expression in early log phase (OD600, ∼0.3)
    Open reading frames expressed at higher levels in the Δupp mutant than in strain NCFM
        1564Membrane protein2.051.040.005
        1970Hypothetical protein (49 amino acids)2.031.020.02
    Open reading frames expressed at lower levels in the Δupp mutant than in strain NCFM
        1873Acetate kinase0.49−1.020.0002
        1568Putative surface protein0.48−1.050.0006
        0586Galactose-1-phosphate uridylyltransferase0.47−1.091.06E-06
        1066Putative phosphorylase0.47−1.102.73E-05
        1871Neopullulanase0.36−1.499.41E-05
        1362Hypothetical protein0.31−1.671.41E-05
Gene expression at mid-log phase (OD600, ∼0.8)
    ORFs expressed at lower levels in the Δupp mutant than in strain NCFM
        1357ABC transporter ATPase and permease protein0.499−1.000.004