TABLE 1.

Electroporation efficiency of S. aureus wild-type and hsdR mutant strains transformed with plasmid pGC2 DNA extracted from E. coli DH5α or from S. aureus RN4220

StrainpGC2 from E. coli DH5 αpGC2 from S. aureus RN4220pGC2 from E. coli DH5α (heat-treated competent cells)c
Transformation efficiency (CFU/μg DNA)aSD (CFU/μg DNA)Transformation efficiency (CFU/μg DNA)bSD (CFU/μg DNA)Transformation efficiency (CFU/μg DNA)bSD (CFU/μg DNA)
RN42203.7 × 1052.3 × 1056.1 × 1053.8 × 1052.0 × 1034.7 × 102
NCTC8325-41.72.63.0 × 1040.2 × 1040.00.0
NCTC8325-4ΔHsdR3.46.23.9 × 1041.9 × 1048.1 × 1013.6 × 101
NCTC8325-4ΔC-term HsdR2.52.81.9 × 1040.3 × 1041.0 × 1039.7 × 102
SH1000007.0 × 1042.6 × 10400
SH1000ΔHsdR002.7 × 1040.3 × 1043.0 × 1010
COL003.0 × 1014.2 × 10100
COLΔHsdR005.0 × 1014.2 × 10100
  • a Average values for six electroporation experiments.

  • b Average values for two electroporation experiments.

  • c S. aureus competent cells were incubated at 56°C for 2 min prior to electroporation.