TABLE 1.

Plasmids used in this study

PlasmidDescriptionSource or reference
pBluescript KSApr, Co1E1 repliconStratagene
pUC18Apr, Co1E1 replicon54
pMP190Cmr, IncQ, promoter probe vector44
pUC4KApr, pBR322 repliconAmersham Pharmacia
pMP77Cmr, IncQ, promoter probe vector44
pRK2013Kmr, Tra+ Mob+, Co1E1 replicon12
pPH1J1Gmr IncP11
pSUP2021Apr Cmr Tcr Kmr pBR325 replicon, broad-host-range vector43
pLAFR3Tcr, broad-host-range cloning vector, IncP145
pQF50Apr, broad-host-range vector, pRO1600 replicon11
pLIR5Tcr, pLAFR3 containing cepIR locus in a 9-kbp EcoRI fragmentThis study
pBIRApr, pBluescript KS containing cep1R locus in a 9-kbp EcoRI fragmentThis study
pCQS1Tcr, pLAFR3 containing B. cepacia DNAThis study
pCQS2Tcr, pLAFR3 containing B. cepacia DNAThis study
pCQS3Tcr, pLAFR3 containing B. cepacia DNAThis study
pLIR::Tn53Tcr, Kmr, pLIR5 with a Tn5 insertion in cepRThis study
pLCIKmTcr, Kmr, pLIR5 with a Kmr cassette in cepIThis study
pSCR1Apr, pQF50 containing PcepI-lacZ and cepRThis study
pMPIRCmr, pMP77 containing the cepIR locus in a 4.5-kbp PstI fragmentThis study
pRPC-1Apr, pUC18 containing a 300-bp PCR fragment of rpoS geneThis study
pCOSRPOS-2CTcr, pLAFR3 containing B. cepacia DNAThis study
pRBS-2Apr, pBluescript KS containing a 5-kbp SmaI fragment from pCOSRPOS-2CThis study
pRBS-3Apr, pBluescript KS containing a 1-kbp PstI fragment from pCOSRPOS-2CThis study
pRBS-3KmApr Kmr, pRBS-3 with a Kmr cassette in the rpoS geneThis study
pCOSRPOS-2CKmTcr Apr Kmr, pCOSRPOS-2C with a Kmr cassette in the rpoS geneThis study
pRPR2Cmr, PrpoS-lacZ fusion in pMP190 promoter probe vectorThis study