TABLE 1.

Specificity of detection of Campylobacter species and non-Campylobacter organisms with the real-time PCR assay

OrganismSourceaSerotype(s)bStrain or reference no.CTΔRn
Campylobacter jejuni NCTC21116823.40.94
NCTC61139225.10.95
Human23212/9123.40.90
Bovine1087880/9524.91.59
Bovine1387878/9528.31.17
Chicken1450359/9628.40.89
Chicken2450821/9824.30.89
Chicken6450254/9630.40.60
Chicken11450361/9629.70.52
Chicken38, 5788086/9530.40.50
Lamb1450888/9822.21.96
Lamb2450749/9824.61.43
MilkND450098/9824.01.05
SandND87024/9525.40.72
SandND77124/9430.40.80
Campylobacter coli NCTCND11366>45.0−0.01
Chicken28450788/98>45.0−0.14
Chicken56450733/98>45.0−0.02
Lamb56450761/98>45.0−0.10
Lamb56450862/98>45.0−0.05
Porcine4987209/95>45.0−0.04
Porcine5387207/95>45.00.07
Porcine5387208/95>45.00.11
Campylobacter lari NCTC11352>45.0−0.03
Shellfish77779/94>45.00.00
Campylobacter lari Shellfish77715/94>45.0−0.05
Campylobacter lari UPTCcNCTC11928>45.0−0.05
Shellfish77625/94>45.0−0.04
Shellfish77716/94>45.00.10
Campylobacter upsaliensis NCTC11540>45.00.01
Campylobacter helveticus NCTC12470>45.0−0.15
Campylobacter fetus subsp. fetusNCTC5850>45.0−0.33
Campylobacter fetus subsp. venerealisNCTC10354>45.0−0.02
Campylobacter hyointestinalis NCTC11609>45.0−0.09
Campylobacter mucosalis NCTC11000>45.0−0.03
Campylobacter sputorum subsp. bubulusNCTC11367>45.0−0.08
Campylobacter sputorum biovar faecalisNCTC11416>45.0−0.03
Campylobacter hyoilei NCTC12884>45.0−0.10
Helicobacter cinaedi NCTC12423>45.0−0.02
Arcobacter butzleri NCTC12481>45.0−0.02
Chicken450427/98>45.0−0.01
Chicken450555/98>45.0−0.01
Chicken450556/98>45.0−0.08
Aeromonas hydrophila NCTC8049>45.00.00
Bacillus cereus NCTC7464>45.00.01
Bacillus subtilis NCTC10400>45.00.01
Clostridium perfringens NCTC8237>45.0−0.02
Escherichia coli NCTC10418>45.0−0.01
Escherichia coli O157, nontoxigenicNCTC12900>45.0−0.02
Escherichia coli O157 VTECdNCTC12079>45.00.02
Lactobacillus casei NCTC10302>45.00.02
Lactococcus lactis NCTC662>45.0−0.05
Pseudomonas aeruginosa NCTC10662>45.0−0.01
Salmonella enteritidis NCTC400452/97>45.00.02
Salmonella enterica serovar TyphimuriumNCTC12023>45.0−0.01
Shigella sonnei NCTC8574>45.0−0.05
Enterococcus faecalis NCTC775>45.0−0.02
Staphylococcus aureus NCTC6571>45.00.02
Coagulase-negative staphylococcusNCTC170454/97>45.00.03
Vibrio parahaemolyticus NCTC10885>45.0−0.05
Vibrio alginolyticus NCTC12160>45.0−0.08
Vibrio cholerae non-O:1NCTC11348>45.0−0.04
Yersinia enterocolitica NCTC10460>45.00.00
Proteus mirabilis NCTC10975>45.0−0.05
Citrobacter freundii NCTC9750>45.0−0.08
Legionella pneumophila NCTC12821>45.0−0.08
Acinetobacter lwoffii NCTC5866>45.00.01
Enterobacter aerogenes NCTC10006>45.00.03
Enterobacter cloacae NCTC11936>45.00.05
Proteus rettgeri NCTC7475>45.0−0.01
Klebsiella pneumoniae NCTC350324/97>45.0−0.02
  • a NCTC, National Collection of Type Cultures, PHLS Colindale; all other isolates were obtained from the Campylobacter Collaborating Unit, Preston Public Health Laboratory.

  • b Campylobacter Penner heat-stable serotype. ND, not determined.

  • c UPTC, urease-positive thermophilic Campylobacter.

  • d VTEC, verocytotoxin-producing E. coli.