TABLE 2.

Detection of C. jejuni in food enrichment cultures by real-time PCR and subculture to selective agar

Sample no.SampleSourceConcordantaCulture resultbPhenotypic identificationReal-time PCR assaycTotal viable Campylobacter countsd
GE-PCRGE-mlΔRnCT
1ThighChickenY+ C. jejuni 2.0 × 1072.7 × 1093.7321.8ND
2ThighChickenY+ C. jejuni 1.3 × 1071.7 × 1093.0122.5ND
3ThighChickenY+ C. jejuni 6.2 × 1068.3 × 1082.8223.6ND
4ThighChickenY+ C. jejuni 7.4 × 1069.8 × 1082.5923.3ND
5DrumstickChickenY+ C. jejuni 1.4 × 1061.9 × 1081.6626.1ND
6DrumstickChickenY+ C. jejuni 2.6 × 1063.5 × 1081.9625.0ND
8Diced meatTurkeyY+ C. jejuni 4.4 × 1065.9 × 1081.8924.2ND
11LiverPigY+ C. jejuni 1.1 × 1031.5 × 1051.6537.5ND
16ShellfishMusselsY+ C. lari −0.0245.0ND
17LiverOxY+ C. jejuni 1.2 × 1061.6 × 1081.9126.3ND
18LiverOxY+ C. jejuni 2.0 × 1052.7 × 1072.0229.2ND
19LiverPigY+ C. jejuni 5.6 × 1057.5 × 1071.7227.5ND
20LiverPigY+ C. jejuni 1.0 × 1061.3 × 1082.3226.5ND
21DrumstickChickenY+ C. jejuni 1.7 × 1052.3 × 1071.5529.4ND
22DrumstickChickenY+ C. jejuni 4.7 × 1036.3 × 1051.4635.1ND
23ThighChickenY+ C. jejuni 1.0 × 1071.3 × 1092.3922.8ND
24ThighChickenY+ C. jejuni 2.1 × 1072.8 × 1093.0221.6ND
25QuarterChickenY+ C. jejuni 2.2 × 1042.9 × 1061.5232.7ND
28MeatTurkeyY+ C. jejuni 4.1 × 1045.5 × 1060.7831.7ND
30WingsChickenY+ C. jejuni 9.4 × 1031.3 × 1061.1229.0ND
31WingsChickenY+ C. jejuni 2.2 × 1052.9 × 1071.6024.4ND
32QuarterChickenY+ C. jejuni 4.9 × 1026.5 × 1040.9833.3ND
33MeatTurkeyY+ C. jejuni 3.8 × 1055.1 × 1071.4823.7ND
35LiverChickenY+ C. jejuni 2.1 × 1042.8 × 1061.2227.9ND
36LiverChickenY+ C. jejuni 1.0 × 1041.3 × 1061.2828.9ND
37DrumstickChickenY+ C. jejuni 5.8 × 1017.7 × 1030.4936.4ND
7MeatLambY0.1945.0
9LiverLambY0.2945.0
10LiverOxY0.1145.0
12MeatPorkY0.3945.0
15ShellfishCocklesY0.0745.0
27MeatTurkeyY0.4045.0
29MeatPorkY0.3645.0
34LiverPigsY0.3645.0
43QuarterChickenY0.0845.0
47ThighChickenY0.1745.0
48ThighChickenY0.0745.0
49DrumstickChickenY0.0645.0
50DrumstickChickenY0.2845.0
51ShellfishCocklesY0.2845.0
52ShellfishCocklesY0.0945.0
59LiverLambY0.0845.0
60LiverPigsY0.3645.0
63KidneyLambY0.2445.0
69MilkRawY0.1845.0
71MilkRawY0.2545.0
73MilkRawY0.3145.0
74MilkRaw (spiked)Y0.1545.0
77MilkRawY−0.0845.0
78MilkRaw (spiked)Y0.0445.0
80MilkRaw (spiked)Y−0.0145.0
82MeatPorkY0.3245.0
91LiverPigsY0.0145.0
92DrumstickChickenY0.3945.0
65ThighChickenN4.0 × 1015.3 × 1031.0937.0
13LiverChickenN4.7 × 1026.3 × 1040.8838.8
38DrumstickChickenY+ C. jejuni 1.1 × 1051.5 × 1071.2525.5ND
39LiverLambsY+ C. jejuni 4.4 × 1045.9 × 1060.7426.8ND
40LiverOxY+ C. jejuni 2.8 × 1063.7 × 1082.4420.8ND
44QuarterChickenY+ C. jejuni 5.8 × 1047.7 × 1061.5526.4ND
45WingsChickenY+ C. jejuni 4.3 × 1055.7 × 1071.7123.5ND
46WingsChickenY+ C. jejuni 3.4 × 1054.5 × 1072.2523.8ND
54DrumstickChickenY+ C. jejuni 2.1 × 1062.8 × 1082.0621.21.0 × 106
55DrumstickChickenY+ C. jejuni 6.1 × 1058.1 × 1071.9623.01.0 × 106
56DrumstickChickenY+ C. jejuni 5.9 × 1047.8 × 1061.6823.01.0 × 106
57DrumstickChickenY+ C. jejuni 1.3 × 1061.7 × 1081.6221.91.0 × 106/PICK>
61LiverOxY+ C. jejuni 9.0 × 1051.2 × 1082.6822.4>6 × 106
62LiverPigsY+ C. jejuni 4.8 × 1056.4 × 1072.7023.31.0 × 106
64MeatTurkeyY+ C. jejuni 5.0 × 1046.7 × 1061.1026.61.0 × 106
66DrumstickChickenY+ C. coli 0.3345.01.0 × 105
67DrumstickChickenY+ C. jejuni 1.1 × 1041.5 × 1061.9228.81.4 × 105
68DrumstickChickenY+ C. jejuni 7.8 × 1051.0 × 1083.4022.62.0 × 105
70MilkRaw (spiked)Y+ C. jejuni 2.0 × 1052.7 × 1071.6724.6>3 × 106
72MilkRaw (spiked)Y+ C. jejuni 6.6 × 1058.8 × 1072.4722.8>1 × 107
76MilkRaw (spiked)Y+ C. jejuni 2.2 × 1012.9 × 1030.8237.83 × 104
81MeatTurkeyY+ C. jejuni 2.5 × 1023.3 × 1040.5434.3<1.0 × 105
84LiverChickenY+ C. jejuni 2.2 × 1022.9 × 1040.9634.42 × 105
85ThighChickenY+ C. jejuni 5.6 × 1047.5 × 1061.0826.4>4 × 105
86DrumstickChickenY+ C. jejuni 3.4 × 1054.5 × 1071.5623.8>1 × 107
87ThighChickenY+ C. jejuni 7.3 × 1059.8 × 1072.2522.7>1 × 107
88MilkPasteurized (spiked)Y+ C. jejuni 7.9 × 1051.1 × 1081.4722.6>1 × 107
89MilkPasteurized (spiked)Y+ C. jejuni 1.9 × 1062.5 × 1081.9721.3>1 × 107
90LiverOxY+ C. jejuni 3.9 × 1055.2 × 1071.4023.6>1 × 107
94DrumstickChickenY+ C. jejuni 1.5 × 1012.0 × 1030.5838.43.0 × 105
95ThighChickenY+ C. jejuni 2.9 × 1043.9 × 1061.0327.46 × 107
96ThighChickenY+ C. jejuni 1.1 × 1061.5 × 1084.1222.29 × 107
93DrumstickChickenN3.24.3 × 1020.6440.6
53ShellfishCocklesN3.04.0 × 1020.4540.7
14LiverChickenN7.5 × 1011.0 × 1040.7441.8
97ThighChickenN5.87.7 × 1020.9241.9
75MilkRawN1.01.3 × 1020.7142.3
79MilkRawN5.4 × 1017.2 × 1030.5343.2
83LiverPigsN+ C. coli 5.3 × 1017.1 × 1030.4843.21.4 × 106
42LiverPigsN+ C. coli 2.1 × 1012.8 × 1030.4544.6ND
41LiverPigsN+ C. jejuni 0.2745.0ND
58LegChickenN+ C. jejuni 0.0645.01.6 × 106
26MeatTurkeyN+ C. jejuni 0.0845.0ND
  • a Concordant: Y, yes; N, no.

  • b Results of subculture to selective agar medium.

  • c GE-PCR, genome equivalents per real-time PCR reaction; GE-ml, genome equivalents per milliliter of enrichment culture.

  • d Total viable Campylobacter count on selective agar after 48 h of enrichment culture (CFU milliliter of enrichment culture). ND, not determined.