TABLE 2.

Growth of S. thermophilus after 24 h under multiple amino acid omissionsa

Amino acid composition in CDM (g/liter)OD600
All amino acids1.55
No amino acids0b
Only His (0.15) and Cys (0.39)0.6c
Only His, Cys, Glu (0.4)0.69
Only His, Cys, Glu, Phe (0.28)0.75
Only His, Cys, Glu, Ser (0.34)0.83
Only His, Cys, Glu, Ala (0.24)0.44
Only His, Cys, Glu, Val (0.33)0.72
Only His, Cys, Glu, Phe, Ser0.73
Only His, Cys, Glu, Phe, Ala0.53
Only His, Cys, Glu, Phe, Val0.61
Only His, Cys, Glu, Ser, Ala0.69
Only His, Cys, Glu, Ala, Val0.82
  • a Data shown are the averages of three parallel cultures. Additional data for these amino acid omission experiments are shown (see Table S1 in the supplemental material).

  • b Negative control (should be 0).

  • c Growth after 48 h.