TABLE 1.

Primers used in this studya

Primer nameSequenceaRestriction site
egfpfwCATATGGTGAGCAAGGGCGAGGAGNdeI
CL2GTGGATCCTTACTTGTACAGCTCGTCBamHI
papdAfwCTCGAGGAGCCTCTCCATTAAACAATGXhoI
papdArevCATATGCTTGAATTCCTCCAACCGGGGGTATGNdeI
prplKfwAAGCTTGGCATCAAGGTTTCGGAAGHindIII
prplKrevCATATGTTTACCCTACCTCTGGTCGNdeI
prpsJfwCTCGAGGTCCTTCG GGATCGCTTG ACXhoI
prpsJrevCATATGATTCACGTCATCCGTTAAATCNdeI
purerevCATATGGTGGTTATGCGCTGCTCAAAATCNdeI
purefwCTCGAGCTTTTCTCGATCCGGGAAAAATACXhoI
PmamDCrevCATATGCTGATCTCCGGCAAGTGTATGNdeI
PmamDCfwCTCGAGCAATGACCACCACCACCTTA AACXhoI
PmamABfwCTCGAGATGGCGCAAAGATGTGACGT CXhoI
PmamABrevCATATGTCCCGTCACAATTCACCTCCNdeI
pmsp3fwCTCGAGGAACTCCAAAAGCAAGGCTATTTACXhoI
pmsp3revATTAATCCGAAAGCTCCTTGAATCAAAAGVspI
PmamDCxlrevCTCGAGGATCTCCGGCAAGTGTATGCACXhoI
zzfwGCTGCACATATGGCGCAACACGATGAAGCCNdeI
zzrevCCATCTAGAAATATTACCGCCAGCCATTGXbaI
  • a Restriction sites that were incorporated in the primer are indicated in bold.