TABLE 1.

Data generated from a standard curve using with RotorGene2000 systema

Oocyst ConcnCV (%)Ct
GivenCalculated
100,00083,93716.0617.34
10,00011,68116.8120.51
1,0001,0262.5924.42
1001087.9128.04
10100.4031.87
117.4935.69
  • a DNA standards consist of serial dilutions of C. parvum DNA at concentrations of 105 to 10° (one) oocysts. The given concentration is the total number of oocysts added to the PCR; the calculated concentration is the oocyst concentration calculated by using RotorGene 2000 software to find a line of best fit. CV, coefficient of variation. The line of best fit variation. The line determined for the standards had the following values: R2, 0.99691; slope, −0.259; y intercept, 9.504.