Oocyst Concn | CV (%) | Ct | |
---|---|---|---|

Given | Calculated | ||

100,000 | 83,937 | 16.06 | 17.34 |

10,000 | 11,681 | 16.81 | 20.51 |

1,000 | 1,026 | 2.59 | 24.42 |

100 | 108 | 7.91 | 28.04 |

10 | 10 | 0.40 | 31.87 |

1 | 1 | 7.49 | 35.69 |

↵

*a*DNA standards consist of serial dilutions of*C. parvum*DNA at concentrations of 10^{5}to 10° (one) oocysts. The given concentration is the total number of oocysts added to the PCR; the calculated concentration is the oocyst concentration calculated by using RotorGene 2000 software to find a line of best fit. CV, coefficient of variation. The line of best fit variation. The line determined for the standards had the following values:*R*^{2}, 0.99691; slope, −0.259;*y*intercept, 9.504.