TABLE 3.

Recovery of pmoA from soil spiked with pure culturesa

Recovered pure culture (assay)Mean no. of target molecules in individual extractionbNo. of:
1234Target moleculesCellsc
CalculatedAdded
Methylosinus trichosporium (TYPEII)0.9 ± 0.10.7 ± 0.10.3 ± 0.10.4 ± 0.10.6 ± 0.10.3 ± 0.1d1.5 ± 0.3 (n = 11)
Methylococcus capsulatus (MCOC)1.5 ± 0.54.6 ± 0.82.2 ± 0.63.1 ± 0.62.9 ± 0.71.4 ± 0.3e1.6 ± 0.2 (n = 13)
Methylomicrobium album (MBAC)0.7 ± 0.20.7 ± 0.10.2 ± 0.10.9 ± 0.10.6 ± 0.20.3 ± 0.1e0.4 ± 0.1 (n = 13)
  • a Soil was spiked with cells of Methylosinus trichosporium, Methylococcus capsulatus, or Methylomicrobium album.

  • b All values are means ± SEs and represent target molecules or cells (107) per milliliter of spiked soil slurry.

  • c Numbers of cells were calculated from mean molecule target numbers; 2 pmoA copies per cell were assumed.

  • d Calculated cell numbers deviated significantly from added cell numbers (P < 0.01).

  • e Calculated numbers of cells were not significantly different from added numbers of cells (P > 0.005).