TABLE 1.

Evidence for growth and sulfate reduction in batch cultures of four SRB grown lithotrophically and heterotrophicallya

Species and growth phaseLithotrophic growth characteristicHeterotrophic growth characteristic
Organism characteristicsPressure (lb/in2)pHOD600Protein concn (mg/ml)Sulfate concn (mM)Sulfide concn (mM)Organism characteristicsPressure (lb/in2)pHOD600Protein concn (mg/ml)Sulfate concn (mM)Sulfide concn (mM)
Desulfovibrio desulfuricansGram-negative mixotroph with CO2-acetateGram-negative, incomplete oxidizer
    Initial15.17.090.068 ± 0.0000.02 ± 0.00021.3b ± 1.0ND10.06.490.049 ± 0.0050.11 ± 0.0119.9 ± 0.80.4 ± 0.3
    Final 18.27.450.164 ± 0.0024.17 ± 0.240.0 ± 0.017.7 ± 3.710.86.720.224 ± 0.0081.74 ± 0.0413.9 ± 0.24.3 ± 0.1
    Final 27.77.400.167 ± 0.0014.09 ± 0.130.0 ± 0.018.7 ± 0.910.96.720.229 ± 0.0282.18 ± 0.2814.0 ± 0.44.7 ± 0.3
Desulfotomaculum acetoxidansGram-positive autotroph, acetyl-CoA pathwayGram-positive, complete oxidizer, acetyl-CoA pathway
    Initial15.17.070.041 ± 0.0000.03 ± 0.0019.0 ± 0.3ND10.06.540.033 ± 0.0030.15 ± 0.0118.4 ± 0.20.3 ± 0.0
    Final 18.77.370.142 ± 0.011ND4.4 ± 1.1ND8.76.950.131 ± 0.004ND13.6 ± 0.2ND
    Final 29.97.380.264 ± 0.0163.18 ± 0.185.3 ± 1.012.0 ± 0.79.06.930.221 ± 0.0101.64 ± 0.1613.6 ± 0.23.4d ± 0.3
Desulfobacter hydrogenophilusGram-negative autotroph, modified TCA cycleGram-negative, complete oxidizer, modified TCA cycle
    Initial15.16.960.060 ± 0.0000.03 ± 0.0019.2 ± 0.4ND10.06.430.097 ± 0.0020.04 ± 0.0018.4 ± 0.80.7 ± 0.0
    Final 17.77.390.378 ± 0.0134.28 ± 0.260.1 ± 0.119.4 ± 0.29.87.070.360 ± 0.0170.86 ± 0.047.8 ± 0.87.4 ± 0.5
    Final 2c15.27.100.037 ± 0.0020.38 ± 0.2818.4 ± 0.70.0 ± 0.09.87.090.381 ± 0.0181.87 ± 0.088.4 ± 0.76.4 ± 0.3
Desulfobacterium autotrophicumGram-negative autotroph, acetyl CoA pathwayGram-negative, complete oxidizer, acetyl-CoA pathway
    Initial14.76.980.068 ± 0.0000.04 ± 0.0218.1 ± 0.2ND10.06.410.090 ± 0.0010.27 ± 0.0218.8 ± 0.40.4 ± 0.1
    Final 16.47.410.884 ± 0.0014.65 ± 0.240.1 ± 0.022.0 ± 0.59.87.070.344 ± 0.0082.32 ± 0.098.5 ± 1.57.8 ± 0.3
    Final 26.37.390.424 ± 0.0024.66 ± 0.140.1 ± 0.020.8 ± 3.09.87.050.359 ± 0.0150.72 ± 0.098.0 ± 1.47.5 ± 0.7
  • a Sterile controls, under lithotrophic- and heterotrophic-growth conditions (respectively), were unchanged in pH (7.09 ± 0.01 and 6.51 ± 0.01), optical density (OD) (0.013 ± 0.003 and 0.014 ± 0.003), protein concentration (0.03 ± 0.00 and 0.01 ± 0.00 mg/ml), sulfate concentration (19.79 ± 0.82 and 22.53 ± 1.99 mM), sulfide concentration (0.44 ± 0.28 and 0.88 ± 0.20 mM), acetate concentration (0.0 ± 0.0 and 10.5 ± 2.6 mM), and pressure (15.0 ± 1.2 and 9.6 ± 0.2 lb/in2) over the duration of the incubation (380 and 168 h). ND, not determined.

  • b Sample was compromised before sulfate was analyzed. Value shown is for an uninoculated control harvested at the same time.

  • c This culture became oxidized due to stopper failure and did not grow.

  • d Values are from a sample taken before substrate degradation was complete.