TABLE 1.

Parameters of linear regressions of manual versus automated counting at different thresholds of the FISH image

FISH threshold (% avg gray value)SlopeRegression parameter y interceptr2
1100.82522.610.939
1201.0202.910.987
1301.025−0.390.989
1401.012−1.440.988
1500.994−1.780.987
1600.978−1.870.986
1700.963−1.880.984
1800.947−1.800.983
1900.934−1.730.982
2000.929−2.280.984