TABLE 2.

Screening of three environmental libraries for genes encoding coenzyme B12-dependent glycerol and diol dehydratases

LibrarySample siteAverage insert size (bp)No. of E. coli clones tested by:No. of dehydratase-positive E. coli clones by:
Activity-based screeningSequence-based screeningActivity-based screeningSequence-based screening
IRiver Grone3,30080,00032,0001 (pAK8)2 (pAK6-7)
IISugar beet field5,000240,00065,0001 (pAK2)2 (pAK4-5)
IIISolar Lake3,400240,00061,00001 (pAK3)