Physical, chemical and biological characterization of Yellowstone hot spring sampling sites

Study site, and specific locationSample typepHTemp (°C)SO4 concn (mM)SRR (nmol of SO4 cm−3 day−1)Phospholipid content (nmol of P g−1)Enrichments obtainedNo. of clades found with DSR PCR amplification of samples fromf:
Mud Volcano area, Obsidian PoolSediment6.3890.6104 (144)b9.7 (3.7)e1
Nymph Creek area
    Nymph CreekScraped Mat2.538-474.7212 (110)n.a.a2
Black Sediment PoolSediment6.6690.687 (97)n.a.3
Mammoth Springs
    New Pit SpringMat6.15631.611,111 (1,497)n.a.+01
    Bath Lake VistaMat6.5546.3n.a.n.a.+21
    Roland's WellMat6.552n.a.n.a.n.a.+11
Norris Geyser Basin
    Site CSediment2.38887.5704cn.a.0
    Site DSediment2.2426.51.6 (1.4)n.a.0
    Site ESediment4.1910.3b.d.d3.9 (3.1)
    Cinder PoolSediment2.5910.52c2.4 (1.2)0
    Black SpringSediment3.0802.65.6c1.8 (0.6)0
Mt. Washburn area
    Site ASediment2.24654.0b.d.25.8 (1.8)
    Site BSediment6.18139.1b.d.n.a.
    Acid Inkpot SpringSediment2.87738.2b.d.6.9 (5.1)
    Inkpot SpringSediment6.28624.1b.d.1.3 (0.1)
  • a n.a., data not available.

  • b Values represent means (standard deviations). n = 3 for all samples except those from New Pit, for which n = 2.

  • c The rate was determined from one core; the other two cores gave results below the detection limit.

  • d b.d., below detection limit.

  • e −, enrichment was attempted with no success.

  • f Values represent the number of clades present. Zeros represent unsuccessful attempts at amplification. DSR, dissimilatory sulfite reductase.