TABLE 3.

Microbiological isolation and direct PCR detection of Campylobacter populations in inoculated bovine feces

Taxon and treatmentaMean ± SEM log CFU g−1 (fresh wt) (n = 3)bMean ± SEM proportion of samples found positive by PCR for:
GenuscC. coli and C. jejunidC. fetus and C. lanienaeeC. hyointestinalisf
Campylobacter coli
    A8.19 ± 0.0431.01.0g
    B7.21 ± 0.0381.01.0
    C6.27 ± 0.0611.01.0
    D5.28 ± 0.0561.01.0
    E4.17 ± 0.0841.01.0
    F3.28 ± 0.0870.50 ± 0.290.83 ± 0.17
    G1.98 ± 0.4900.67 ± 0.170.0
    N0.00.50 ± 0.290.0
Campylobacter fetus
    A7.97 ± 0.0301.01.0
    B7.09 ± 0.0651.01.0
    C6.04 ± 0.0371.01.0
    D5.08 ± 0.0151.01.0
    E4.07 ± 0.0041.01.0
    F3.13 ± 0.1031.00.50 ± 0.29
    G2.48 ± 0.0590.33 ± 0.330.17 ± 0.17
    N0.00.33 ± 0.170.0
Campylobacter hyointestinalis
    A7.73 ± 0.0801.01.0
    B6.52 ± 0.1291.01.0
    C5.58 ± 0.1571.01.0
    D4.79 ± 0.0131.01.0
    E3.67 ± 0.0371.01.0
    F2.95 ± 0.0511.00.67 ± 0.33
    G2.13 ± 0.0690.50 ± 0.290.50 ± 0.29
    N0.00.67 ± 0.330.0
Campylobacter jejuni
    A8.14 ± 0.0711.01.0
    B7.24 ± 0.0291.01.0
    C6.21 ± 0.0531.01.0
    D5.28 ± 0.0301.01.0
    E4.28 ± 0.0121.01.0
    F3.38 ± 0.0910.83 ± 0.170.67 ± 0.17
    G2.49 ± 0.1140.67 ± 0.170.16 ± 0.17
    N0.00.83 ± 0.170.0
  • a Treatments consisted of inoculation of feces with different concentrations of the target bacterium (10-fold dilutions); treatment N was uninoculated (control).

  • b Feces content ranged from 19.1% ± 0.80% to 19.8% ± 0.36% dry matter.

  • c Nonnested Campylobacter genus-specific primer set targeting the 16S rRNA gene.

  • d Nested multiplex primer sets for C. coli and C. jejuni targeting the ceuE and mapA genes, respectively.

  • e Nested multiplex primer sets for C. fetus and C. lanienae targeting the 23S and 16S rRNA genes, respectively.

  • f Seminested primer set for C. hyointestinalis targeting the 23S rRNA gene.

  • g —, not determined.