TABLE 3.

Identities of bands obtained from the bacterial community of Stilton cheese as a result of DGGE analysis of the V4-V5 region

BandaOriginClosest relative% IdentityGenBank accession no.
1Stilton fingerprintb Lactococcus lactis 99 AF498065
3Stilton fingerprint Staphylococcus sp.99 AF498066
4Stilton fingerprint S. equorum 100 AF498069
6Stilton fingerprint Leuconostoc mesenteroides 99 AF498080
2Bulk cells on M17 agar (dilution, 10−7) E. faecalis 100 AF498081
3Bulk cells on M17 agar (dilution, 10−7) Staphylococcus sp.99 AF498067
4Bulk cells on M17 agar (dilution, 10−7) S. equorum 100 AF498073
6Bulk cells on M17 agar (dilution, 10−7) Leuconostoc mesenteroides 99 AF498079
3Bulk cells on MRS agar (dilution, 10−7) Staphylococcus sp.99 AF498068
4Bulk cells on MRS agar (dilution, 10−7) S. equorum 100 AF498072
6Bulk cells on MRS agar (dilution, 10−7) Leuconostoc mesenteroides 100 AF498076
6Bulk cells on anaerobic MRS agar (dilution, 10−7) Leuconostoc mesenteroides 100 AF498077
5Bulk cells on Rogosa agar (dilution, 10−6) Lactobacillus plantarum 100 AF498075
4Bulk cells on MSA (dilution, 10−6) S. equorum 100 AF498070
4Bulk cells on nutrient agar (dilution, 10−6) S. equorum 100 AF498070
6Bulk cells on nutrient agar (dilution, 10−6) Leuconostoc mesenteroides 100 AF498078
  • a The numbers of the bands refer to those in Fig. 2.

  • b DGGE pattern obtained after PCR amplification of DNA directly extracted from Stilton cheese.