TABLE 1.

Germination and hyphal growth of T. harzianum strains on media with combinations of antimicrobial compounds

MediumaGerminationcDiam of T. harzianum colonies (mm)b from group (genotype):
1 (Th1)2 (Th7)
MEAYes85d85d
1Yes49 (±0.4)37 (±0.3)
2Yes79 (±0.8)68 (±0.4)
3No17e (±0.9)48e (±1.9)
4No28 (±0.4)20 (±2.0)
TSMNo9.3 (±0.25)8.2 (±0.54)
  • a Media: MEA, 2% (wt/vol) malt extract medium; medium 1, 2% MEA, chloramphenicol, streptomycin, and sodium deoxycholate; medium 2, 2% MEA, chloramphenicol, and streptomycin; medium 3, 2% MEA, chloramphenicol, streptomycin, and nystatin; medium 4, 2% MEA, chloramphenicol, streptomycin, captan, and propamocarb.

  • b Mean diameters of colony growth are given, with standard errors of the means from 5 replicates in parentheses.

  • c Germination was either totally inhibited or was unaffected.

  • d Colony diameters of both T. harzianum groups on media 1 to 4 and TSM were significantly different when compared with MEA controls by analysis with the two-tailed paired t-test, P > 0.05.

  • e Colony growth was only detected after day 12 on medium 3; all other measurements were recorded on day 3. Colony diameters of the two groups on medium 3 are significantly different when analyzed with a two-tailed paired t-test P > 0.05.