TABLE 2.

Quantification of the various bacterial components known to produce butyric acid within the human fecal microbiotaa

TargetProbebNo. of cells/g (dry wt) and percentage of the total microbiota in volunteer no.b
12345678910
All eubacteriaUniversal11.711.811.611.812.211.71211.711.511.9
Ruminococcus-Eubacterium-Clostridium clusterErec48210.7 (5.2)10.7 (8.06)11 (26.4)10.5 (5.2)10.9 (5.3)10.9 (15)10.8 (6.3)10.6 (8.4)10.8 (19.7)10.9 (8.8)
E. hallii clusterEhal5788.8 (0.13)10.1 (2)8.3 (0.03)9.1 (0.1)9 (0.17)10.4 (2.4)8.8 (0.12)9.2 (0.48)9 (0.12)
R. intestinalis clusterRint6039.6 (0.87)10.4 (3.88)10 (2.6)10 (1.66)10.6 (2.2)10 (2.07)10.4 (2.74)9.8 (1.22)10.2 (4.98)10 (1.16)
R. intestinalisRint11028.4 (0.05)8.4 (0.03)9.5 (0.44)9.5 (0.22)9.4 (0.47)8.5 (0.06)8.8 (0.2)9.2 (0.2)
Eubacterium sp. strains L1-83 and A2-1948.4 (0.03)10.1 (1.28)9.1 (0.24)9.4 (0.51)8.8 (0.2)9.4 (0.26)
E. rectale sp. strain A1-86Erec2078.8 (0.12)8.5 (0.06)8.5 (0.04)9.5 (0.34)
E. ramulusEram9978.5 (0.05)8.1 (0.03)8.9 (0.05)8.9 (0.01)8 (0.02)8.6 (0.05)
Coprococcus sp. strain L2-508.8 (0.035)
E. hadrumEhad5799.1 (0.4)9.1 (0.2)8.5 (0.1)9 (0.14)9.3 (0.01)9.5 (0.56)9.2 (0.17)9.2 (0.2)
E. ventriosumEvent6610.1 (1.7)10.2 (1.7)
E. cylindroides groupEcyl38710.2 (2.5)9.9 (1.7)
E. biformeEbif46210.2 (2.5)9.9 (1.7)
F. prausnitzii clusterFpra65510.1 (2.7)10.5 (4.6)10.2 (4.1)10.4 (3.75)10.8 (3.6)10.5 (5.53)10.1 (1.35)10.5 (5.89)10.2 (4.9)10.2 (1.8)
  • a All probes were negative for E. barkeri, E. multiforme, E. moniliforme, E. saburreum, E. cylindroides, E. dolichum, E. limosum, A. caccae, C. lituseburense, and C. histolyticum.

  • b Counts of bacteria are expressed as numbers of organisms log10 per gram of feces (dry weight). Coefficient of variation due to assay error of the fluorescent in situ hybridization method was 0.18 (13). The percentage of the total microbiota was calculated by using counts from the universal eubacterial probe (1H).