TABLE 3.

Properties of the BLIS produced by S. suis serotype 2

Property or strain testedInhibitory activity or resistance shown by producing strain:
94-62390-1330AAH465
Production in
    Todd-Hewitt agar plates+a+++
    Todd-Hewitt agar plates + CaCO3++++
    Todd-Hewitt broth+++b
    LA broth+++
Sensitivity to
    100°C, 15 minRcRRNDe
    121°C, 20 minSdSSND
    pH 2.0RRRND
    pH 12.0RRRND
    TrypsinRRRND
    ChymotrypsinRRRND
    ElastaseSSSND
    Proteinase KSSSND
    PronaseSSSND
Dialysis (12-14 kDa)f+++ND
Ultrafiltration (30 kDa)g+++ND
Activity spectrum for bacteria
    Gram-positive
        E. durans DM++++
        E. faecalis ATCC 19433
        E. faecalis ATCC 29212
        E. hirae ATCC 8043
        M. luteus ATCC 272++++
        S. hyicus ATCC 11249
        S. bovis ATCC 9809
        S. bovis B457C+
        S. agalactiae ATCC 13813++++
        S. dysgalactiae subsp. dysgalactiae ATCC 27957++++
        S. dysgalactiae subsp. dysgalactiae ATCC 9926+
        S. equi subsp. zooepidemicus ATCC 6580++++
        S. equi subsp. zooepidemicus ATCC 43079
        S. dysgalactiae subsp. equisimilis ATCC 9542++++
    Gram-negative
        A. minor AMX 2B++++
        A. porcinus 96-0088-3F++++
        H. parasuis 99-9048-B
        P. multocida 01-14-97-2
  • a +, inhibitory activity.

  • b −, no inhibitory activity.

  • c R, resistant.

  • d S, sensitive.

  • e ND, not determined.

  • f BLIS activity in dialysis tubing.

  • g BLIS activity in ultrafiltrates.