TABLE 2.

Fluorescently labeled oligonucleotide probes used for in situ hybridization experiments

ProbeTargetSequence (5′ to 3′)% FMaSpecificitySource or reference
EUB33816SGCT GCC TCC CGT AGG AGT35Bacteria domain1
ALF96816SGGT AAG GTT CTG CGC GTT20α-Proteobacteria43
ACD63816SCTC AAG ACA ACA CGT CTC20Acidiphilium spp.This work
BET42a23SGCC TTC CCA CTT CGT TT35β-Proteobacteria39
GAM42a23SGCC TTC CCA CAT CGT TT35γ-Proteobacteria39
THIO116SGCG CTT TCT GGG GTC TGC35Group a Acidithiobacillus spp.M. Stoffels, unpublished data
ACT465a16SGTC AAC AGC AGC TCG TAT20Group b Acidithiobacillus spp.This work
ACT465b16SGTC AAC AGC AGA TCG TAT10Group c Acidithiobacillus spp.This work
ACT465c16SGTC AAC AGC AGA TTG TAT10Group d Acidithiobacillus spp.This work
ACT465d16SGTC AAT AGC AGA TTG TAT10Acidithiobacillus spp.This work
NTR712b16SCGC CTT CGC CAC CGG CCT TCC35Nitrospira group14
LEP15416STTG CCC CCC CTT TCG GAG35Leptospirillum ferriphilumThis work
LEP63416SAGT CTC CCA GTC TCC TTG40Group a Leptospirillum spp.This work
LEP63616SCCA GCC TGC CAG TCT CTT35Group c Leptospirillum ferrooxidansThis work
LEP43916SCCT TTT TCG TCC CGT GCA35Group c1 Leptospirillum ferrooxidansThis work
LEP43216SCGT CCC GAG TAA AAG TGG20Group c2 Leptospirillum ferrooxidansThis work
SRB38516SCGG CGT CGC TGC GTC AGG35δ-Proteobacteria1
DSS65816STCC ACT TCC CTC TCC CAT60Desulfosarcina, Desulfococcus, Desulfofrigus40
DSV69816SGTT CCT CCA GAT ATC TAC GG35Desulfovibrio spp.40
ACM116016SCCT CCG AAT TAA CTC CGG35Acidimicrobium spp.This work
ARCH91516SGTG CTC CCC CGC CAA TTC CT20Archaea domain55
FER65616SCGT TTA ACC TCA CCC GAT C35Ferroplasma spp.16
TMP65416STTC AAC CTC ATT TGG TCC35Thermoplasma spp., Picrophilus spp.This work
NON338ACT CCT ACG GGA GGC AGC35Negative control1
  • a Percent (vol/vol) formamide in the hybridization buffer.

  • b The name of this probe in the work of Daims et al. (14) is S-*-tspa-0712-a-A-21.