TABLE 5.

DAPI and FISH counts with bacterial-, group-, and species-specific probes

Sampling site and date (mo yr)Total cell counts (105 ml−1)Fraction (%) of cells detected with probea:
EUB338ALF968ACD638BET42aGAM42aTHIO1ACT465aACT465bACT465cACT465dNTR712LEP634LEP154LEP636LEP439LEP432ACM1160TMP654FER656
RT1
    Oct. 9914 ± 592 ± 22 ± 0.3BDLbBDL52 ± 151 ± 245 ± 2BDLBDLBDL40 ± 13 ± 0.36 ± 132 ± 128 ± 1BDLBDLBDLBDL
    May 005 ± 181 ± 1BDLBDLBDL21 ± 121 ± 533 ± 1BDLBDLBDL55 ± 12 ± 0.35 ± 0.437 ± 0.338 ± 1BDLBDLBDL2 ± 1
RT2
    Oct. 994 ± 281 ± 455 ± 242 ± 2BDL<1BDLBDLBDLBDLBDL2 ± 0.2BDLBDLBDLBDLBDLBDLBDLBDL
    May 003 ± 174 ± 13 ± 0.1BDL35 ± 0.41 ± 0.12 ± 0.11 ± 0.1BDL1 ± 0.2BDL3 ± 0.11 ± 0.1BDL9 ± 0.51 ± 0.13 ± 0.2BDLBDLBDL
RT3
    Oct. 9916 ± 684 ± 22 ± 0.2BDL81 ± 2BDLBDLBDLBDLBDLBDLBDLBDLBDLBDLBDLBDL1 ± 0.3BDLBDL
    May 004 ± 183 ± 1BDLBDL64 ± 1BDLBDLBDLBDLBDLBDLBDLBDLBDLBDLBDLBDL1 ± 0.3BDLBDL
RT4
    Oct. 997 ± 389 ± 216 ± 111 ± 18 ± 0.539 ± 225 ± 121 ± 12 ± 0.2BDLBDL30 ± 1BDL1 ± 0.235 ± 135 ± 1BDL4 ± 1BDLBDL
    May 0014 ± 378 ± 3BDLBDL2 ± 0.13 ± 0.3BDL1 ± 0.3BDLBDLBDL42 ± 22 ± 0.1BDL34 ± 15 ± 0.533 ± 0.2BDL<1<1
RT5
    Oct. 993 ± 185 ± 217 ± 118 ± 1BDL35 ± 220 ± 119 ± 12 ± 0.3BDLBDL22 ± 1BDL1 ± 0.333 ± 120 ± 1BDL5 ± 1BDLBDL
    May 0014 ± 381 ± 1BDLBDL4 ± 0.23 ± 0.1BDL3 ± 0.3BDL1 ± 0.1BDL51 ± 1BDL1 ± 0.159 ± 18 ± 0.543 ± 1BDL<1<1
RT6
    Oct. 99NDNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
    May 0013 ± 483 ± 11 ± 0.1BDLBDL48 ± 150 ± 146 ± 312 ± 129 ± 1<121 ± 0.5<11 ± 0.19 ± 0.37 ± 0.3<11 ± 0.3BDL2 ± 0.2
RT7
    Oct. 9965 ± 1077 ± 127 ± 1BDLBDLBDLBDLBDLBDLBDLBDL50 ± 12 ± 0.1BDL47 ± 13 ± 0.252 ± 13 ± 0.3BDLBDL
    May 0082 ± 1285 ± 1<1<11 ± 0.169 ± 0.170 ± 21 ± 0.146 ± 129 ± 0.42 ± 0.37 ± 0.4<1BDL8 ± 0.52 ± 0.15 ± 0.3<1<1BDL
RT8
    Oct. 9945 ± 1080 ± 120 ± 0.419 ± 15 ± 135 ± 135 ± 13 ± 0.210 ± 0.36 ± 0.3BDL7 ± 0.20.5 ± 0.1BDL3 ± 0.2BDL0.4 ± 0.1BDLBDLBDL
    May 005 ± 177 ± 115 ± 0.314 ± 0.43 ± 123 ± 115 ± 0.32.3 ± 0.18 ± 0.46 ± 0.31 ± 0.84 ± 0.21 ± 0.1<15 ± 0.23 ± 0.21 ± 0.1BDL<13 ± 0.1
RT9
    Oct. 99150 ± 1785 ± 116 ± 12 ± 0.2BDL59 ± 173 ± 2<126 ± 0.514 ± 1<11 ± 0.1BDLBDLBDLBDLBDLBDLBDLBDL
    May 00140 ± 1985 ± 1BDLBDLBDL64 ± 170 ± 112 ± 156 ± 110 ± 0.3<119 ± 0.4NDNDNDNDNDBDLBDLBDL
RT10
    Oct. 9916 ± 586 ± 128 ± 0.613 ± 1BDLBDLBDLBDL1 ± 0.1BDLBDL65 ± 1BDLBDL69 ± 1BDL64 ± 1BDLBDLBDL
    May 009 ± 283 ± 18 ± 0.213 ± 0.572 ± 1<1<1BDLBDL<1BDL<1BDL<1BDLBDLBDLBDLBDLBDL
  • a Percent detection compared to detection of all DAPI-stained cells. ND, not done.

  • b BDL, below detected limit (<0.1).