TABLE 3.

Quantitative comparison of the architectures of wild-type V. cholerae and ΔvpsL mutant biofilms formed in three model seawater mediaa

MediumStrainBiomass (μm3/μm2)Fraction of substratum covered by cellsAvg thickness (μm)Maximum thickness (μm)
ASW/CAAWild type0.76 ± 0.010.59 ± 0.060.96 ± 0.075.39 ± 0.24
ΔvpsL0.45 ± 0.010.62 ± 0.020.48 ± 0.042.62 ± 0.03
DSW/CAAWild type0.29 ± 0.020.44 ± 0.030.41 ± 0.052.0 ± 0.48
ΔvpsL0.23 ± 0.050.41 ± 0.050.30 ± 0.051.76 ± 0.25
RSW/CAAWild type0.41 ± 0.080.42 ± 0.050.55 ± 0.086.66 ± 0.4
ΔvpsL0.40 ± 0.020.45 ± 0.080.51 ± 0.055.64 ± 1.77
  • a Biofilms were formed in either commercial artificial seawater supplemented with CAA (ASW/CAA), seawater prepared from defined components supplemented with CAA (DSW/CAA), or seawater from the Massachusetts coast supplemented with CAA (RSW/CAA). Values were calculated by using the Comstat program (13).