TABLE 1.

Reactivities of MAbs (as measured by the OD450) against A. anophagefferens and a diverse array of microalgae, protozoa, and bacteriaa

Microbial taxonOD450Microbial taxonOD450
Pelagophytes
    Aureococcus anophagefferens (WNB)0.96
    Aureococcus anophagefferens (GSB)1.03
    Aureococcus anophagefferens (BB)0.97
    Aureoumbra lagunensis<0.01
    Pelagococcus subviridis<0.01
Cyanobacteria
    Synechocystissp. (Syn 145)0.03
    Synechococcus sp. (8012)<0.01
    Synechococcus sp. (8101)<0.01
Prochlorophytes (Prochlorococcus sp.)<0.01
Cryptophytes (Rhodomonas salina [3C])0.01
Chlorophytes
    Ankistrodesmus falcatus<0.01
    Chlamydomonassp.0.01
    Chlorella stigmatophora (CL993)<0.01
    Chlorella capsulata (Fla-E)<0.01
    Nanochloris sp. (Nanno)<0.01
Prasinophytes
    Tetraselmis sp.<0.01
    Micromonas pusilla (IB4)<0.01
Prymnesiophytes
    Prymnesiophyte (H3-14)<0.01
    Isochrysis galbana (T-Iso)<0.01
    Chrysochromulina ericina (NEPCC109A)<0.01
Chrysophytes
    Paraphysomonas bandaiensis (Hflag)0.01
    Paraphysomonas imperforata (VS1)<0.01
    Ochromonassp. (VT1)0.04
Bacillariophytes
    Thalassiosira weissflogii (Actin)<0.01
    Nitzschia frustulem0.01
    Minutocellus polymorphus0.01
Pedinellids (Pteridomonas sp. [NB1])<0.01
Synurophytes (Synura petersenii)0.01
Bicosoecids (Cafeteria sp. [Cflag])0.02
Hamatores (Caecitellus parvulis)0.02
Dictyophytes (Rhizochromulinasp.)<0.01
Raphidophytes (Heterosigma akashiwo)0.01
Xanthophytes (Botrydiopsis intercedens)0.03
Jakobids (Jakoba libera [DB9])<0.01
Eustigmatophytes
    Eustigmatos magna0.02
    Nannochloropsis gaditana<0.01
Euglenids (Euglena gracilis)<0.01
Dinoflagellates
    Gymnodinium beii0.01
    Scrippsiella sp.0.02
Ciliates
    Scuticociliate (BBcil)0.03
    Hymenostome (Hcil)<0.01
Bacteria (Halomonas halodurans)0.01
Unidentified algae
    Long Island mixture (LI16a)*0.01
    Long Island mixture (LI15a)*<0.01
    BT3 (unidentified, 2- to 3-μm alga)*<0.01
    BT8 (unidentified, 2- to 3-μm alga)*<0.01
  • a Values of <0.01 were undetectable under the conditions employed. Cultured A. anophagefferens from three locales were tested (WNB, West Neck Bay, New York; GSB, Great South Bay, New York; BB, Barnegat Bay, New Jersey; see Materials and Methods for strain identifications). Asterisks indicate unidentified taxa (verified as not A. anophagefferens) of similar size and shape to the brown tide alga that were enriched and cultured from natural water samples with a high abundance of A. anophagefferens. OD450, optical density at 450 nm.