TABLE 2.

Difference alignment of the target region of the 16S rRNA for LKC-specific probes

Probe and targetTarget sequencea
LKC59 probe sequence (5′-3′)TCCTCTCATCGTTCGACT
    Target sequenceAGUCGAACGAUGAGAGGA
    LKC group I clonesb------------------
    Uncultured Cesspool Cave clone CC-4 (AF207530)-------------U----
    Uncultured groundwater clone 1023 (AB030610)-------------U----
LKC1006 probe sequence (5′-3′)CTCCAATGTTTCCATCGG
    Target sequenceCCGAUGGAAACAUUGGAG
    Most LKC group II clonesc------------------
    LKC1.114_5 (AY191480)---G--------------
    LKC1.199_5 (AY191494)-U----------------
    LKC1.199_6 (AY191495)---------U--------
  • a —, identical to the probe sequence.

  • b Based on nine LKC group I clones with 16S rRNA gene sequences at this position.

  • c Based on 15 LKC group II clones.