TABLE 2.

Bacterial 16S rDNA sequences obtained from natural gas pipeline samples

Closest relative in GenBank (accession no.)No. of sequences derived from DGGE bands for sample:% Identity
S3S4S2S5B1B2B2RA1A2
Acetobacterium psammolithicum (AF132739 )196
Acidovorax delafieldii isolate N7-28 (AF332187 )1100
Acinetobacter junii (AF417863 )211197-100
Acinetobacter sp. (DSM590) (X81659 )199
Acinetobacter sp. (ATCC17924) (Z93443 )198
Acinetobacter sp. strain AU1523 (AY043369 )199
Anaerofilum pentosovorans (X97852 )11198
Bacteroides sp. strain 253c (AY082449 )1299
Citrobacter werkmanii (AF025373 )199
Clostridium acidisoli strain CK74 (AJ237756 )192
Clostridium algidixylanolyticum (AF092549 ) 198
Clostridium butyricum strain E5 (AJ458419 )1298-100
Clostridium sp. (Y12289 )199
Clostridium sp. strain IrT-JG1-67 (AJ295658 )199
Comamonas denitrificans strain 110 (AF233876 )23121296-100
Desulfovibrio aminophilus (AF067964 )2195-98
Desulfovibrio desulfuricans (AF354664 )199
Desulfovibrio sp. strain zt10e (AF109469 )194
Enterococcus casseliflavus strain CECT969T (AJ420804 )1100
E. coli (AF511430 )11636196-99
E. coli O157:H7 (AE005489 )199
E. coli O39:NM (AY078065 )299-100
Geotoga aestuarianus strain T3B (AF509468 )299-100
Halanaerobium congolense (U76632 )199
Klebsiella pneumoniae (AF453251 )195
Klebsiella pneumoniae (AF511429 )1299-100
Klebsiella pneumoniae (X87276 )199
Microbacterium sp. strain 44574 (AY082801 )199
Microbacterium testaceum strain CE648 (AF474330 )198
Peptostreptococcus indolicus (D14147 )199
Propionibacterium sp. strain V07/12348 (Y17821 )297
Propionibacterium sp. strain WJ6 (AY096033 )11100
Pseudomonas aeruginosa strain H13 (AY074894 )1299-100
Pseudomonas iners (AB021408 )199
Pseudomonas sp. strain NZ066 (AF388209 )199
Pseudomonas sp. strain PM-2001 (AF321239 )1100
Pseudomonas stutzeri strain DNSP21 (U26414 )198
Pseudomonas stutzeri strain YPF-41 (AJ410872 )199
Ralstonia sp. strain MBIC3838 (AB007998 )199
Rhizobium sp. strain RM1-2001 (AF331662 )199
Sporomusa ovata (DSM2662) (AJ279800 )298-99
Staphylococcus auricularis (ATCC33753T) (D83358 )199
Sulfurospirillum sp. strain 18.1 (AF357199 )391-92
Thermus thermophilus HB27 (L09659 )1100
Uncultured bacterium clone GOUTA13 (AY050603 )193
Uncultured bacterium clone IA-23 (AJ488074 )294-95
Uncultured bacterium clone KRA30-58 (AY081994 )199
Uncultured bacterium clone RB13C11 (AF407414 )1198-99
Uncultured bacterium mle1-42 (AF280863 )189
Uncultured beta proteobacterium clone 1518 (AF467353 )1100
Uncultured eubacterium clone GL182.6 (AY038619 )11198-99
Unidentified eubacterium clone vadinHA73 (U81735 )189
Total71515971217915