TABLE 2.

Quantitative comparison of the architectures of wild-type V. cholerae biofilms formed in various model freshwater mediaa

MediumBiomass (μm3/μm2)Fraction of substratum covered by cellsAvg thickness (μm)Maximum thickness (μm)
10 mM NaCl/YT1.93 ± 0.660.58 ± 0.062.83 ± 1.0210.19 ± 1.15
10 mM NaCl/CAA0.11 ± 0.020.08 ± 0.010.16 ± 0.021.49 ± 0.31
10 mM NaCl/CAA/CRB1.85 ± 0.270.42 ± 0.052.17 ± 0.3111.38 ± 0.69
CRW/CAA0.4 ± 0.060.37 ± 0.040.45 ± 0.062.64 ± 0.84
  • a Biofilms were formed in either 10 mM NaCl supplemented with YT (10 mM NaCl/YT), 10 mM NaCl supplemented with CAA (10 mM NaCl/CAA), 10 mM NaCl supplemented with CAA and monosaccharides (10 mM NaCl/CAA/CRB), or Charles River water supplemented with CAA (CRW/CAA). Values were calculated by using the Comstat program (13).