TABLE 2.

Archaeal 16s rDNA and mcrA clone recovery from MOA-containing sediments and fosmid DNA librarya

DNAGroupRecovery (no. of clones)Total no. of clones
Eel RiverMonterey Canyon F17.1AMIS microcosm C4.1Blake Ridge PC26
T201Gzfos
Archaeal 16s rDNAANME-16281374
ANME-2
ANME-2a14142
ANME-2b99
ANME-2c201274391
Methanogen-like112224
Other64931591
mcrA groupa13492551
b+6+ND6
c34314529
d134
e33
  • a T201 environmental sequences and the Gzfos library were both derived from the same purified cell preparation of MOA from Eel River T201 sediment (see text). Environmental samples and fosmid library were screened with universal mcrA primers ME-1 and ME-2 and ANME-1 type b-specific mcrA primers AOM39 and AOM40 (see text). +, positive amplification with AOM39 and AOM40 primer set. mcrA group represents conceptual translation product. ND, not determined.