TABLE 1.

Bacterial strains, plasmids, and PCR primers used in this study

Strain, plasmid, or primerRelevant characteristic(s) or sequenceSource or reference
V. fischeri
    ES114Wild-type light-organ isolate from E. scolopes5
    ESR1Spontaneous Rfr mutant of ES11414
    JRM100 to JRM104Five ES114 clones with mini-Tn7 insertion; EmrThis study
    JRM200ES114 clone with mini-Tn7 insertion; CmrThis study
E. coli
    BW23474Δlac-169 robA1 creC510 hsdR514 uidAMluI)::pir116 endA(BT33) recA128
    CC118λpirΔ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-I rpsE rpoB argE(Am) recA1; lysogenized with λpir16
Plasmids
    pEVS94SR6K ori oriT Emr41
    pEVS104Derivative of pRK2013; conjugal tra and trb genes41
    pEVS107pEVS94S derivative, mini-Tn7; mob; Emr KnrThis study
    pUXBF13R6K ori; tns genes; Apr4
    pCR2.1PCR product cloning vector; ColE1 ori; Apr KnrInvitrogen Inc.
    pJRM3pEVS107 with Cmr replacing EmrThis study
    pEVS35Cmr XbaI fragment cloned into XbaI site of pBC39
Primers
    JM15′ GTTACACGTTACTAAAGGG 3′This study
    JM25′ ACCAGACCGTTCAGCTGG 3′This study
    JM35′ GGAACATGTGTGGTATGG 3′This study
    JM45′ GACAGTCATCTATTCAAC 3′This study