TABLE 4.

Microorganisms detected in Samotlor water samples by the microchip method

Targeted organism(s)No. of probes hybridizing with DNA of sampleIdentification by culture-based methodsa
13044757Injection water
Thermococcus + Pyrococcus6, 306, 30b+
Desulfurococcus44
Methanobacterium + Methanothermobacter5, 585, 585, 58+c
Thermotoga + Thermosipho545454+
Geotoga21
Petrotoga25, 27+
Thermoanaerobacter505050+
Desulfotomaculum I464646+c
Desulfotomaculum II4848+c
Thermodesulfobacterium39
Aquificales + Desulfurobacterium + Thermovibrio555555d
Geobacillus53+
Thermus5252
  • a +, identification successful; −, identification failed.

  • b —, hybridization at background level.

  • c Morphotypes similar to known representatives of these genera were observed in enrichment cultures.

  • d No cultivation attempts were made for these organisms.